Human Serum Albumin (HSA, 66 kDa) typically accounts for 65% or more of the total protein present in serum and plasma samples. This can prevent the detection of low abundant plasma and serum proteins in most experiments. Albumin also distorts lanes during electrophoresis if a large amount of total protein is loaded to permit the detection of low abundant proteins. Albumin can also mask other proteins that migrate around 50-70 kDa thereby preventing their detection.
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